Second-half goals from Jo Kuffour and Chris Lines dealt a blow to Colchester's promotion hopes as Bristol Rovers ran out 3-2 winners.
Rovers went ahead after 16 minutes through Dominic Blizzard's shot from 25 yards, but Colchester hit back five minutes later when John-Joe O'Toole's header deflected a Paul Ifil shot past Mikkel Andersen.
Kuffour forced the ball home from Lines' header after 67 minutes, and then provided the cross for Lines to make it 3-1 with an 80th-minute volley.
Colchester substitute Kayode Odejayi shot home from 12 yards with injury time approaching, and in a frantic finish Andersen saved well at the feet of Kevin Lisbie.
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